Separation of lipids by Silica Gel G column chromatography.
نویسندگان
چکیده
A column chromatographic procedure utilizing Silica Gel C is described for separating lipid components of serum and lipoproteins into individual fractions containing hydrocarbons (I), cholesterol esters (II), triglycerides (111), cholesterol (IV) , free fatty acids (V) , and phospholipids (VI). Silica Gel G required no pretreatment except adjustment of moisture content to 10%. The method affords a rapid, complete separation of all major lipid classes except diglycerides. Recoveries of serum and tissue phospholipids were approximately 60-80%, whereas those of the other major lipid classes were essentially quantitative.
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ورودعنوان ژورنال:
- Journal of lipid research
دوره 5 3 شماره
صفحات -
تاریخ انتشار 1964